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Beamline 2-ID-E: 2-ID-E Microprobe
X-ray Science Division, APS
Life Sciences, Environmental Science, Materials Science
Description
Station 2-ID-E houses an x-ray fluorescence microprobe with XRF tomography and XRF laminography capability at submicron resolution
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Supported Techniques
- Microfluorescence
- Tomography
- Laminography
- Ptychography
Beamline Controls and Data Acquisition
The beamline is run using LINUX workstations using various EPICS-based applications. A user interface allows simple operation for users with little prior beamline experience. Custom software MAPS and XRF-Maps allows analysis of full fluorescence spectra at each pixel.
Detectors
- Silicond Drift Detectors Vortex ME7 (7 element)
- Ionization chambers
- Eiger 500
Additional Equipment
- Leica DMXRE Epi-fluorescence microscope
- X-ray alignment camera
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Local Contacts
Beamline Specs
Source (upstream) |
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Source (downstream) |
3.3 Undulator (Undulator A) |
Monochromator Type |
Kohzu-Si(111) |
Energy Range |
5-20 keV |
Resolution (ΔE/E) |
1.4 x 10 -4 |
Flux (photons/sec) |
4 x 10 9 @10 keV |
Beam Size (HxV) |
Focused |
.5µm x .4µm
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Unfocused |
2mm x 2mm
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For additional information see:
https://www.aps.anl.gov/Microscopy/Beamlines/2-ID-E
Current Status:
Commissioning
Access Mode:
On-site
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Selected Publications
- Dodani, S.C., D.W. Domaille, C.I. Nam, E.W. Miller, L.A. Finney, S. Vogt and C.J. Chang (2011) "Calcium-dependent copper redistributions in neuronal cells revealed by a fluorescent copper sensor and X-ray fluorescence microscopy." PNAS, 108(15): p. 5980-5.
- Kim, A., S. Vogt, T. O'Halloran, T. Woodruff (2010). "Zinc Availability Regulates Exit from Meiosis in Maturing Mammalian Oocytes". Nature Chemical Biology, 6(9): 674-81
- Holzner, C., M. Feser, S. Vogt, B. Hornberger, S. B. Baines, C. Jacobsen (2010). "Zernike Phase Contrast in Scanning Microscopy with X-rays". Nature Physics, 6(11): p. 883-887.
- Wolford, J.L., Y. Chishti, Q. Jin, J. Ward, L. Chen, S. Vogt, L. Finney (2010) "X-ray Fluorescence Microscopy Reveals Direct Correlation Between Increase of Nuclear Zinc and Loss of Pluripotency in Human Embryonic Stem Cells". PLoS ONE, 5(8): e12308
- de Jonge, M.D., C. Holzner, S.B. Baines, B.S. Twining, K. Ignatyev, J. Diaz, D.L Howard, A. Miceli, I. McNulty, C.J. Jacobsen, S. Vogt (2010). "Quantitative 3-D elemental microtomography of Cyclotella meneghiniana at 400-nm resolution". PNAS, 107(36): 15676-80.
- Kehr, S., M. Malinouski, L. Finney, S. Vogt, V.M. Labunskyy, M.V. Kasaikina, B.A. Carlson, Y. Zhou, D.L. Hatfield, and V.N. Gladyshev (2009). "X-ray fluorescence microscopy reveals the role of selenium in spermatogenesis" Journal of Molecular Biology 389(5): 808-18.
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