Beamline 18-ID-D: BioCAT

Description

The Biophysics Collaborative Access Team (BioCAT) is an NIH P30 Mature Synchrotron Resource for Structural Biology supported national user facility that runs the Sector 18 beamline for the study of the structure and dynamics of disordered and partially ordered biological systems. Solution scattering (BioSAXS) experiments, which provide data about macromolecular conformation in solution, can be done in equilibrium or in a time-resolved mode with microsecond to second time resolution. Separation coupled modes, such as SEC-SAXS, as well as more traditional batch mode experiments, are supported for BioSAXS. Fiber diffraction patterns of exceptional quality can be obtained from complex, weakly diffracting biological systems in very short exposure times. Part of this fiber program is a unique muscle diffraction program, providing instrumentation and expertise to measure equilibrium and time-resolved patterns from both skinned and intact muscle.

Supported Techniques

• Fiber diffraction
• Small-angle x-ray scattering
• Time-resolved x-ray scattering
• BioSAXS/BioSANS
• Microdiffraction

Detectors

• EIGER2 XE 9M
• Mar 165 CCD detector
• EIGER 500k pixel array detector

Additional Equipment

• Muscle mechanics/X-ray diffraction apparatus
• Small angle camera (up to 5.5 m camera length)
• Coflow SAXS cell
• Continuous-flow and stopped-flow mixers for SAXS
• Combined x-ray cell/muscle physiology apparatus
• Agilent 1260 Infinity II HPLCs
• Wyatt Eclipse AF4
• Wyatt DAWN Helios II Multi-angle light scattering w/ DLS
• Wyatt Optilab T-rex (Refractive Index detector)
• Fully capable wetlab for protein purification and sample and buffer preparation

Local Contacts

Beamline Specs

For additional information see:
https://www.bio.aps.anl.gov/

Current Status:

Access Mode: